Final diagnosis


Botryosphaeria spp. (dieback)

Sectoral lesion in which Botryosphaeriacae was isolated (<b> dieback at <i>Botryosphaeria</i> </b>)
Beginning and superficial lesion visible under the bark of a vine affected by esca. Over time it will be colonized in particular by one to several species of <i> Botryosphaeria </i> (<b> dieback at <i>Botryosphaeria</i> </b>)
Detail of a sectoral lesion of Botryosphaeria (<b> dieback of <i>Botryosphaeria</i> </b>)
Sectorial botryosphaeria canker on one-year-old wood. (<b> dieback at <i>Botryosphaeria</i> </b>)
Cut through a perithecium of <b> <i> Botryosphaeria obtusa </i> </b>, a fungus involved in BDA (black dead arm) disease.
Detail of the globular structures or pycnidia covering this rotten and partially shriveled grape berry. (<i> Neofusicocum parvum </i>).
Rotten and partially shriveled grape berry. Globular and mycelial structures cover the altered tissues. (<i> Neofusicocum parvum </i>).
This ascus of <i> Botryosphaeria parva </i> contains 8 hyaline, non-septate ascospores measuring 18/27 × 8/11 µm. Later, they will turn light brown and show 1 to 2 partitions (teleomorph of <i> Neofusicoccum parvum </i>).
<i> <b> Diplodia serratia </b> </i> conidium forming at the tip of a hyaline conidiophore. The conidia of this fungus are 25/27 × 10/12 µm, and they are brown and without septum (anamorph of <i> <b> Botryosphaeria obtusa </b> </i>)
Conidia of <i> <b> Diplodia mutila </b> </i> measuring 23.5 / 27 × 12/14 µm. They often remain hyaline and without septum, with a thick wall. (anamorph of <i> <b> Botryosphaeria stevensii </b> </i>)
The conidia of <i> Lasiodiplodia theobromae </i> have the following dimensions: 24/28 × 12/15 µm. They are initially hyaline and not septate, and become dark brown, with a septum and irregular longitudinal striations. (anamorph of <i> <b> Botryosphaeria rhodina </b> </i>)
Appearance of globular pycnidia of <i> Neofusicoccum parvum </i>. We can clearly see their circular ostiole.
Formation of the conidia of <i> Neofusicoccum parvum </i> inside a pycnidia.
Appearance of hyaline and non-septate conidia of <i> Neofusicoccum parvum </i>.